False positive (FP) filters are a second layer of scrutiny which can be automatically applied to chromosomes determined by ADCI to be dicentric. When a dicentric chromosome meets the threshold for a FP filter, it will no longer be classified as dicentric for the purposes of dose estimation and will have a yellow contour in the metaphase image viewer. A set of FP filters are described below and can be activated individually. By default, all FP filters are active.
False positive dicentric chromosomes are assigned to one or more of the following morphological classes:
FP filters target one of more of these classes.
To open the FP filter selection dialog, click “Settings” in the menu bar at the top of the software window and select “Change FP Filters”. The dialog appearing on the right side of the screen will appear. A checkmark beside a filter indicates it is active.
When a sample is highlighted within the main GUI a “FP Flag” field is visible. This is a decimal number which encodes which FP filters are active through the use of “bit flags”. A FP Flag value of 0 indicates no FP filters are active. For other FP Flag values, the decimal number must be converted to binary to decipher which FP filters are active.
These calculations are unnecessary if the goal is simply to determine which FP filters are active while ADCI is running. Active filters are displayed in the “Change FP Filters” dialog discussed above. Deciphering FP filters based on the FP Flag may be necessary if console output is saved and viewed at a later date. If active FP filters have been forgotten during that time, the FP Flag present in the console output can be used to recover them.
Many online tools exist to convert a decimal to binary such as this Decimal to Binary Converter. Simply input the “FP Flag” value and click “Convert”. The Windows built-in calculator can also perform this conversion by selecting “View” → “Programmer”, then selecting “Dec” using the radio button.
In this example, a FP Flag of 78 was shown after highlighting a sample in the main GUI. In binary the decimal number 78 is equivalent to 01001110. Make note of the position of each “1” reading from right to left. In this case, the positions are 2, 3, 4, and 7. Then refer to the list below to discover which FP filter each digit corresponds to. Thus, if the FP Flag is 78, the Chromosome Area, Chromosome Maximum Width, Chromosome Centromere Width, and Intercandidate Concavity Index filters are active.
87654321 - Position. Corresponds to the list of filters directly below.
01001110 - Binary digits.
The 8 bits of interest are as follows: